Begoña Martínez-Cruz1, Janet Ziegle2, Paula Sanz1, Graciela Sotelo1, Roger Anglada3, Stéphanie Plaza3 and David Comas1* for the Genographic Consortium Abstract Background: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe. Results: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%). Conclusions: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions paper is at http://www.investigativegenetics.com/content/pdf/2041-2223-2-13.pdf Table 1 Genetic markers analysed in the OpenArray, the Y haplogroup they define, the alleles and their call rates (successful genotype calls) List of genetic markers analysed many HG listed by Marker Haplogroup Alleles Call rate % Bob I2*, ISOGG new I2b, I2c DNA Project Tyler Family DNA Project Genealogy Sunshine Coast ISOGG Papers/Presentations Cited A Fair Deal for Members of the Dafence and Ex Service Community Resgstore the Parity with CENTRELINK and other DVA Pensions that the ALP Govt stole from Disabled Veterans's Disability Payment The march to CANBERRA has begun. Will YOU join us?