Regarding Glenda's observations of allele 464... 1. I, too, will await a response from Rolla regarding the technical aspects. 2. I concur with the writer, John Chandler, regarding the example of why the names of donors who are close relatives should not be released until or unless they have reviewed the results of YDNA tests and concur with the release of their name. 3. The 3 Queen DNA samples with mutations on 464c do NOT appear to be anything other than coincidental mutations - except for the possibility of my John R. Queen. If there is a "problem" in his lineage, it clearly appears to have been an adoption issue. It is hoped that more samples will resolve this issue. Gene

Greetings Queen listers, I'm back and beginning to work through the 200 emails in my inbox. Seems as if someone started a nasty rumor about my birthday being on May 5th. While I really truly appreciate all the birthday wishes, my birthday is not the 5th of May. Last time I looked, it was the 9th of May. So I will bank those early wishes until my real birthday, which is ...uh....today! Happy Birthday to me! Anyway, I will try to address a couple of questions that came up while I was gone. Up until know we have sort of made believe that each of the 37 DNA markers were distinct and that a change in one marker equalled one mutation. For the most part that is true, but there are a couple of places where the reality is more complicated. This is true of markers labeled 464 a, b, c, d and e and 389 I and II. To determine the number of mutations for these markers you have to compare across the board and not one to one. For 464 the sequence is not important, it is matches between the groups. It is hard to explain, but easy to visualize. Let's pretend we have the following gene sequence from different individuals a b c d e 15 15 17 17 15 15 17 17 There are an equal number of 15s and 17 in the two individuals - so there is no genetic difference. But the following individuals have: 15 17 15 17 17 15 15 17 Although it looks like a mismatch in the first two markers, there are still two 17s and two 15s, so there is no difference between the two. There is no change. The following sequence is: 15 15 16 17 17 15 17 15 In the above the two 15s match and one 17 matches, but there is one mismatch between the 16 and 17, so there would be 1 mutation separating these two individuals. With the 389 I and II genes, the comparisons are similarly quirky. Look at the following examples 389 I and 389 II 15 16 15 16 The above would be a perfect genetic match. But I and II are redundant pairs, and as long as the mutation shifts between the numbers are parallel, the change is counted as 1 mutation. 15 16 16 17 would represent a 1 (not 2) step mutation since there is a parallel shift or increase the each loci of 1. Look a the results of the Group 8 Queens for an example of what appears to be two mismatches - but only 1 mutation. but 15 16 16 18 are not equal shifts. This would represent a 2 step mutation. This is all quick and dirty, and there are more comprehensive explanations on the web. But this is a start. My apologies on the mistake reporting the 26707 results. I just missed the mutation. I will post corrected results in in a while. Rolla http://webpages.charter.net/rlqueen/DNA/queenmarker.htm http://www.familytreedna.com/public/queenDNA/ http://webpages.charter.net/rlqueen -------Original Message------- From: O Eugene Queen Date: 05/08/05 12:44:20 To: [email protected] Subject: [QUEEN] 464 Mutation Follow-up Regarding Glenda's observations of allele 464... 1. I, too, will await a response from Rolla regarding the technical aspects. 2. I concur with the writer, John Chandler, regarding the example of why the names of donors who are close relatives should not be released until or unless they have reviewed the results of YDNA tests and concur with the release of their name. 3. The 3 Queen DNA samples with mutations on 464c do NOT appear to be anything other than coincidental mutations - except for the possibility of my John R. Queen. If there is a "problem" in his lineage, it clearly appears to have been an adoption issue. It is hoped that more samples will resolve this issue. Gene ==== QUEEN Mailing List ==== Visit Reiley Kidd's homepg to view Queen information compiled and catagorized by first name: http://www.geocities.com/Heartland/Bluffs/2864/ QUEEN YDNA PROJECT http://www.familytreedna.com/surname_join.asp?code=X96855&special=True http://www.ysearch.org/ ============================== Search the US Census Collection. Over 140 million records added in the last 12 months. Largest online collection in the world. Learn more: http://www.ancestry.com/s13965/rd.ashx -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.11.6 - Release Date: 5/6/2005 -- No virus found in this outgoing message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.11.7 - Release Date: 5/9/2005