FIRST RESULTS It seems like the R1b ht35 experts have made very good predictions, however the final outcome is still open. Here are the primary results as we have scored them today: Sample 3064 3804 PF6332 failed C+ PF6333 T+ T+ Y7782 A+ A+ Y7771 T+ T+ V1684 G- failed SK2076 G- G- V3181 failed C- V69 failed C- Y18462 T- T- Y18459 A- A- PF6368 G- G- Z30246 C- C- L388 G- G- M18 del- del- U152 C- C- Unfortunately a few results failed at the first attempt. 3804 / V1684 was likely a clogged capillary and the sample quality of 3064 is generally a little bit lower. I don't think we need to re-run PF6332 since it's implicit positive. However V3181,V69 and V1684 are still interesting and we'll try to solve them in a second sequencing attempt. To get your orientation I recommend to look at the following two trees: R1b-M343 (xM269) Y-DNA tree. v 2. November 25, 2015 (Sergey Malyshev 2015): http://www.kumbarov.com/ht35/R1b-M343xM269%20Y-DNA%20tree_02_11_25_2015.pdf YFull Experimental YTree v3.18 (Vladimir Urasin 2016) http://www.yfull.com/tree/R1b/ (top section, for Y18462 and Y18459) At the moment Vincent Tilroe is still in the lead since he was the first who predicted the R1b-Y7771 level. However, as you can see there are still plenty of phylo-equivalent SNPs at the V3181 level that could be potentially positive. The challenge is now to identify those SNPs out of them that could be available at YSEQ. I suggest you look them up in http://ybrowse.org and check what SNPs are in the proximity. Then you look up those makers with the YSEQ search box if they are available. I will also go through this list later today, but of course you want to be faster than the other participants of this challenge. More risk friendly participants may also check on the V1169, V69 and Y18485 branches. Note that you still have time to pledge for Bonnie's A00 Cameroon project http://experiment.com/a00west until Jan 6th and participate at this challenge. The rules can be found here: http://www.yseq.net/R1bCameroonChallenge.html Good luck! Thomas On 01/04/2016 03:12 AM, Thomas Krahn via wrote: > UPDATE UPDATE UPDATE UPDATE > Thank you! We have had quite a few sponsored SNPs that we'll run > tomorrow morning in the PCR. For simplicity we'll test the same SNPs for > both samples now. The SNPs currently in processing are: > V1684 > L388 > SK2076 > Z30246 > M18 > U152 > PF6333 > PF6332 > PF6292 > Y7782 > PF6368 > V69 > Y18462 > Y18459 > V3181 > Y7771 > Results are expected for Tuesday. Then you'll still have the chance to > bet on markers further downstream from the SNPs that will (hopefully) be > derived. Prepare yourself and do some research. If you want to win, you > must be fast so that you're the first to pledge for a "terminal" SNP. > Note that Wednesday is the last possibility to donate to the Cameroon > A00 project. That's when the last entries for the challenge are accepted. > http://experiment.com/a00west > > On 01/02/2016 10:46 PM, Thomas Krahn via wrote: >> New Year Challenge: R1b in Cameroon >> >> Among our samples collected by Mathew on his last field trips we have >> found the following two haplotypes: >> >> http://www.yseq.net/R1bCameroonChallenge.html >> >> Note that Y-GATA-H4 is in the NIST standard. If you want to compare them >> to FTDNA you need to subtract 1. The haplogroup predictors clearly claim >> 100% R1b. >> >> Now the question is: How did those two Y chromosomes find their way to >> Sub-Saharan Africa? Could it be that some Europeans have left their >> traces during colonization times? >> >> Well, if you're familiar with R1b in Europe, the haplotypes still look a >> little bit strange. >> >> Also we keep finding R1b distribution maps that highlight an R1b >> hot-spot in Northern Cameroon: >> http://www.eupedia.com/europe/Haplogroup_R1b_Y-DNA.shtml >> https://en.wikipedia.org/wiki/Haplogroup_R1b >> >> Wikipedia characterizes the hotspot in Northern Cameroon as R1b-V88. >> However V88 is in a region of the Y chromosome that is 97.9% identical >> to ChrX, so it wouldn't qualify for a stable marker according to the >> newest ISOGG guidelines. I'd rather prefer to test stable known SNPs >> that independently proof the association with the North African and >> Arabic regions. >> >> I'd like to involve you, the R1b experts to use your knowledge and >> experience to solve this mystery in a group effort. At the same time I'd >> like to give the A00 Cameroon Research Project another push for donations. >> >> So here are the rules of the game: >> >> YSEQ will do free SNP tests on those 2 samples if you donate an equal >> amount ($17.50 per SNP) to Bonnie Schrack's A00 Cameroon Research project. >> >> http://experiment.com/a00west >> >> When you make the donation, send Bonnie and myself an email >> thomas@yseq.net, bonnieschrack@gmail.com with the marker you want to >> sponsor and the YSEQ ID it should be tested for. The marker must be >> available in the YSEQ catalog: >> >> https://www.yseq.net/ >> >> or at least we must have the primers in stock so that we can quickly >> test them. Ask us for a distinct SNP if you're unsure. >> >> We will process the SNPs in the very next batch and release the results >> to the public. You can keep sponsoring the next round when the results >> come in. But note that the A00 Cameroon project deadline at >> experiment.com is on January 6th! So there are only 5 days left to >> submit your suggested SNP and the associated sample ID. >> >> The Prize: >> >> The researcher who submits the most downstream positive SNP first will >> win a free Haplogroup Panel at YSEQ which he can use for a person of his >> choice. Since there are two samples, there are two Haplogroup Panels >> that you can win! >> >> Good luck and Happy New Year! >> >> Thomas >> >>

I am not taking part in round two but for those who are, following is a joker: Target SNP Available at YSEQ Y:14710648 (A/C) A7751 Y:19406232 (A/G) Z6297 Y:22181512 (C/G) Z32749 Y17712 CTS2840 Many of the V-series SNPs definitions are not available in YBrowse. And by the way Thomas, there still are unnamed SNPs in YBrowse: ChrY point snp 14724064 14724064 . + . ChrY point snp 8823895 8823895 . + . ChrY point snp 16455403 16455403 . + . ChrY point snp 22478402 22478402 . + . ChrY point snp 23156203 23156203 . + . ChrY point snp 18680627 18680627 . + . ChrY point snp 17491148 17491148 . + . ChrY point snp 21106558 21106558 . + . ChrY point snp 21429550 21429550 . + . Atanas Kumbarov On 05/01/16 19:11, Thomas Krahn via wrote: > FIRST RESULTS > > It seems like the R1b ht35 experts have made very good predictions, > however the final outcome is still open. > Here are the primary results as we have scored them today: > > Sample 3064 3804 > PF6332 failed C+ > PF6333 T+ T+ > Y7782 A+ A+ > Y7771 T+ T+ > V1684 G- failed > SK2076 G- G- > V3181 failed C- > V69 failed C- > Y18462 T- T- > Y18459 A- A- > PF6368 G- G- > Z30246 C- C- > L388 G- G- > M18 del- del- > U152 C- C- > > Unfortunately a few results failed at the first attempt. 3804 / V1684 > was likely a clogged capillary and the sample quality of 3064 is > generally a little bit lower. > I don't think we need to re-run PF6332 since it's implicit positive. > However V3181,V69 and V1684 are still interesting and we'll try to solve > them in a second sequencing attempt. > > To get your orientation I recommend to look at the following two trees: > > R1b-M343 (xM269) Y-DNA tree. v 2. November 25, 2015 (Sergey Malyshev 2015): > http://www.kumbarov.com/ht35/R1b-M343xM269%20Y-DNA%20tree_02_11_25_2015.pdf > > YFull Experimental YTree v3.18 (Vladimir Urasin 2016) > http://www.yfull.com/tree/R1b/ (top section, for Y18462 and Y18459) > > At the moment Vincent Tilroe is still in the lead since he was the first > who predicted the R1b-Y7771 level. > However, as you can see there are still plenty of phylo-equivalent SNPs > at the V3181 level that could be potentially positive. > The challenge is now to identify those SNPs out of them that could be > available at YSEQ. > I suggest you look them up in http://ybrowse.org and check what SNPs are > in the proximity. Then you look up those makers with the YSEQ search box > if they are available. I will also go through this list later today, but > of course you want to be faster than the other participants of this > challenge. > > More risk friendly participants may also check on the V1169, V69 and > Y18485 branches. > > Note that you still have time to pledge for Bonnie's A00 Cameroon > project http://experiment.com/a00west until Jan 6th and participate at > this challenge. > The rules can be found here: > http://www.yseq.net/R1bCameroonChallenge.html > > Good luck! > > Thomas > > > > On 01/04/2016 03:12 AM, Thomas Krahn via wrote: >> UPDATE UPDATE UPDATE UPDATE >> Thank you! We have had quite a few sponsored SNPs that we'll run >> tomorrow morning in the PCR. For simplicity we'll test the same SNPs for >> both samples now. The SNPs currently in processing are: >> V1684 >> L388 >> SK2076 >> Z30246 >> M18 >> U152 >> PF6333 >> PF6332 >> PF6292 >> Y7782 >> PF6368 >> V69 >> Y18462 >> Y18459 >> V3181 >> Y7771 >> Results are expected for Tuesday. Then you'll still have the chance to >> bet on markers further downstream from the SNPs that will (hopefully) be >> derived. Prepare yourself and do some research. If you want to win, you >> must be fast so that you're the first to pledge for a "terminal" SNP. >> Note that Wednesday is the last possibility to donate to the Cameroon >> A00 project. That's when the last entries for the challenge are accepted. >> http://experiment.com/a00west >> >> On 01/02/2016 10:46 PM, Thomas Krahn via wrote: >>> New Year Challenge: R1b in Cameroon >>> >>> Among our samples collected by Mathew on his last field trips we have >>> found the following two haplotypes: >>> >>> http://www.yseq.net/R1bCameroonChallenge.html >>> >>> Note that Y-GATA-H4 is in the NIST standard. If you want to compare them >>> to FTDNA you need to subtract 1. The haplogroup predictors clearly claim >>> 100% R1b. >>> >>> Now the question is: How did those two Y chromosomes find their way to >>> Sub-Saharan Africa? Could it be that some Europeans have left their >>> traces during colonization times? >>> >>> Well, if you're familiar with R1b in Europe, the haplotypes still look a >>> little bit strange. >>> >>> Also we keep finding R1b distribution maps that highlight an R1b >>> hot-spot in Northern Cameroon: >>> http://www.eupedia.com/europe/Haplogroup_R1b_Y-DNA.shtml >>> https://en.wikipedia.org/wiki/Haplogroup_R1b >>> >>> Wikipedia characterizes the hotspot in Northern Cameroon as R1b-V88. >>> However V88 is in a region of the Y chromosome that is 97.9% identical >>> to ChrX, so it wouldn't qualify for a stable marker according to the >>> newest ISOGG guidelines. I'd rather prefer to test stable known SNPs >>> that independently proof the association with the North African and >>> Arabic regions. >>> >>> I'd like to involve you, the R1b experts to use your knowledge and >>> experience to solve this mystery in a group effort. At the same time I'd >>> like to give the A00 Cameroon Research Project another push for donations. >>> >>> So here are the rules of the game: >>> >>> YSEQ will do free SNP tests on those 2 samples if you donate an equal >>> amount ($17.50 per SNP) to Bonnie Schrack's A00 Cameroon Research project. >>> >>> http://experiment.com/a00west >>> >>> When you make the donation, send Bonnie and myself an email >>> thomas@yseq.net, bonnieschrack@gmail.com with the marker you want to >>> sponsor and the YSEQ ID it should be tested for. The marker must be >>> available in the YSEQ catalog: >>> >>> https://www.yseq.net/ >>> >>> or at least we must have the primers in stock so that we can quickly >>> test them. Ask us for a distinct SNP if you're unsure. >>> >>> We will process the SNPs in the very next batch and release the results >>> to the public. You can keep sponsoring the next round when the results >>> come in. But note that the A00 Cameroon project deadline at >>> experiment.com is on January 6th! So there are only 5 days left to >>> submit your suggested SNP and the associated sample ID. >>> >>> The Prize: >>> >>> The researcher who submits the most downstream positive SNP first will >>> win a free Haplogroup Panel at YSEQ which he can use for a person of his >>> choice. Since there are two samples, there are two Haplogroup Panels >>> that you can win! >>> >>> Good luck and Happy New Year! >>> >>> Thomas >>> >>> > > ------------------------------- > To unsubscribe from the list, please send an email to GENEALOGY-DNA-request@rootsweb.com with the word 'unsubscribe' without the quotes in the subject and the body of the message >