Here is a copy paste from Genealogy DNA list; I also viewed directly Thomas' comments on FB. I do not have an opinion as I do not know enough about this technology to have one; even if I knew more I'm not certain that anyone knows all the technical answers. FG (Full Genomes--the citizen scientist owned and operated group) for instance stated they were sending a rep to Hong Kong to discuss delays of the sequencing for their batches and attempt to discover what the problems there are. The message here is that none of the companies and perhaps not geneticists themselves know enough about this technology to fully be able to predict performance, accuracy, or any of the rest of it. There are several with deep pockets and have gone the route of FG who have ordered Big Y. I would guess comparing the results will be the proof to the pudding; unfortunately that will not occur before the price goes up at FTDNA from $495 to $699.00 Krahn write: "Since I have designed this "Big Y" test by myself I can confirm that the enrichment assay is designed to cover about 18 Mb and I took special care for tweaking it so that the final coverage is in fact around 10MB." Here I recall that Wei Wei et al. 2012, to obtain high-quality SNPs, limited the testing to only 8.97 Mbps, after the separation of all insecure regions Y-DNA. Skip exploration in regions repeated. They received an average of about 1029 SNPs per sample. They suggested, however, that in the future you need to take into account the additional regions where there is any possibility of finding qualified polymorphisms. Stan ----- Original Message ----- From: "Brian Swann"<[email protected]> To:<[email protected]> Sent: Sunday, November 10, 2013 10:05 AM Subject: Re: [DNA] FTDNA new Y test Just before we all go overboard, you might want to read what Thomas Krahn has posted at Full Genomes on Facebook: Since I have designed this "Big Y" test by myself I can confirm that the enrichment assay is designed to cover about 18 Mb and I took special care for tweaking it so that the final coverage is in fact around 10MB. This was confirmed by a couple of test runs that have been done at Illumina. No such testing has been done in the FTDNA lab yet. However FTDNA is planning on doing 15000 of those tests on the HiSeq machines which means that they'll combine quite a lot of samples on a single run with "DNA barcoding". I am very worried that this will bring up a lot of crosstalk which will lead to confusing results across the combined samples. David Mittelmann didn't even get the numbers right for the WTY so I doubt that he'll be able to do the correct math for the Next Gen sequencing. In either case customers will be excited about the price and will not care about quality. On 11/10/2013 6:38 AM, Rob McFadden wrote: > I don't think this has been posted on this list yet: > http://cruwys.blogspot.co.uk/2013/11/the-new-big-y-test-from-family-tree-dna.html > > FTDNA is offering a new test for $499 (limited time, then $699) that > covers 10 million base pairs, 25,000 known SNPs and is expected to > discover new SNPs. > > The Full Genome is currently out of my price range. This is low > enough to be tempting, but would be a stretch. Are enough details out > there that someone could explain how much coverage this provides vs. > the Full Genome test? I also would like to know more about the > possibility of discovering new SNPs. If the whole genome is not > covered, what are the odds of finding them? > > Rob > > > ------------------------------- > To unsubscribe from the list, please send an email to [email protected] with the word 'unsubscribe' without the quotes in the subject and the body of the message >