A Busby DNA project, dealing only with Y DNA (males) was announced on this list in June.  Today we have the first report to share with the list.  If you are a male Busby descendant, we would love to have you join us.  The project is ongoing, and will be more helpful to everyone with more participants to evaluate. Report attached.  Wayne and Pat Busbice ********************* REPORT BUSBY DNA SURNAME PROJECT (Busby - a Scottish surname meaning "hamlet in the bushes") by Wayne and Patricia Busbice October 14, 2003 With the recent scientific advances in DNA technology, we have a perfect tool to assist us in the search for ancestors. Genetics teaches us that although human males have both X and Y chromosomes, females carry only X chromosomes. It is the chromosome contribution from the father that determines the sex of the child: XX or XY. Thus the Y chromosome is passed from generation to generation only through the male line, essentially unchanged. The chromosomes mutate very slowly over time, at a predictable rate, allowing identification through DNA of specific individuals as belonging to the same family or line of descent. This rate of mutation, or change, is extremely slow, being measured in terms of 200 to 500 generations. This is the first report based on results from Family Tree DNA of the first group of participants in the twelve marker Busby DNA surname project. We are attempting to verify lineage based on our individual research and to clarify the relationships among our earliest identified ancestors. Further, we are hoping to link up with ancestral lines that go back to Scotland or England by breaking down the genealogical "brick walls" that exist in the 18 century generation of Benjamin, Jeremiah, Zachariah, John, William, Needham and others. This report presents a comparison of the relationship of our first seven participants and state what we have learned to date. However, we refer you to the Family Tree DNA website ([email protected]) if you are interested in finding out more. We now have eight people enrolled in the study, and have received test results on seven. We are still hoping that more Busby/Busbee/Buzby/Busbie/Busbay/Busbice male descendants will join our exciting project, which will continue to be open to all qualified male individuals. Each participant has given permission to publish the relatedness results. Regarding the haplotype and haplogroup data, we will protect the privacy of participants by using a random code for identification. Therefore, no details such as kit or code number, used in the project, are being given which could allow someone to access their genetic information. 12 Marker Genetic Distance Analysis From: WAYNE BUSBICE From: JOHN M. BUSBY Distance Distance John M. Busby 0 Wayne Busbice 0 Roger Busbice 0 Roger Busbice 0 Howard R. Busby 1 Howard R. Busby 1 Michael Lee Busby 2 Michael Lee Busby 2 Dan Eugene Busby 17 Dan Eugene Busby 17 Ernest Lee Busbee, Jr. 19 Ernest Lee Busbee, Jr. 19 From: HOWARD R. BUSBY From: MICHAEL LEE BUSBY Distance Distance Roger Busbice 1 Roger Busbice 2 Wayne Busbice 1 Wayne Busbice 2 John M. Busby 1 John M. Busby 2 Michael Lee Busby 3 Howard R. Busby 3 Dan Eugene Busby 16 Ernest Lee Busbee, Jr. 17 Ernest Lee Busbee, Jr. 18 Dan Eugene Busby 17 From: DAN EUGENE BUSBY From: ERNEST LEE BUSBEE, JR. Distance Distance Ernest Lee Busbee, Jr. 4 Eugene Busby 4 Howard R. Busby 16 Michael Lee Busby 17 Wayne Busbice 17 Howard R. Busby 18 John M. Busby 17 Wayne Busbice 19 Michael Lee Busby 17 John M. Busby 19 Roger Busbice 17 Roger Busbice 19 From: ROGER BUSBICE From: VOHN G. Busby Distance Wayne Busbice 0 report pending John M. Busby 0 Howard R. Busby 1 Michael Lee Busby 2 Dan Eugene Busby 17 Ernest Lee Busbee, Jr. 19 What can we conclude from the above chart? John, Roger and Wayne had a perfect match of 12 of 12 genetic markers which indicates there is a 50% probability their Most Recent Common Ancestor (MRCA) is no longer than 14.5 generations away, a 90% probability of no longer than 48 generations away, and a 95% probability of no longer than 62.4 generations away. However, John, Roger and Wayne know through their own research that t heir MRCA is Benjamin Buzbee, Sr., born 1699 and died 1815. And the DNA test seems to confirm this. In Wayne's case Benjamin, Sr. is back only six generations; seven generations for Roger, and eight for John. Wayne and Roger have traced their lineage from Benjamin, Sr. through his son William Reese Busbee. John recently stated that the DNA results lead him to theorize that Thomas or Jeremiah were also sons of Benjamin, Sr., and that one of them is his direct connection to Benjamin, Sr. Howard Ray Busby's genetic profile was 11 of 12, compared to John, Roger and Wayne. But his personal research revealed that his oldest known ancestor was also Benjamin Buzbee, Sr. From Benjamin Sr. his lineage is through Zachariah born 1768, Jeremiah born 1795, Jeremiah (Jesse) born 1823, Jeremiah (Jerry) born 1856, William H. born 1878. This line places Zachariah as possibly Benjamin Sr.'s oldest son, and a brother to William Reese, a hypothesis that several researchers have been trying to prove. This makes Howard and Wayne fourth cousins twice removed. But what about his genetic distance of 1 from John, Roger and Wayne? The lab explained that the 385 marker is fast-moving and likely to mutate. And when combined with the Busby surname, a less than common name, the mutation is not a problem. This means Howard is related to John, Roger and Wayne as closely as John, Roger and Wayne are related to each other. Michael Lee Busby's genetic marker was 10 of 12 when compared to John, Roger and Wayne and a 50% probability that the MRCA was no longer ago than 61 generations. There are two ways with DNA testing to confirm or deny this. One way is to test additional family members to search for a line that shows a mutation that is one point closer to his sample. The other way is to order the Y-DNARefine 13 marker panel. According to the lab, refining greatly enhances the ability to determine relatedness. Michael Lee has ordered the 25 marker test, as have Roger, John and Wayne, so there will be a comparison available. Michael Lee's 25 marker results have been received and will be compared with the other three men when all reports are available to us. Ernest Lee Busbee, Jr. and Dan Eugene Busby are both separated by three or more genetic markers when compared to other participants in the study. The odds greatly favor their not having shared a common male ancestor with anyone else in the group for more than 2000 years. Hopefully they will be able to convince other possible cousins to join our group to verify their lineage. Apparently everyone except Roger checked the block that stated we wanted our comparisons limited to the Busby surname group participants. Roger authorized Family Tree DNA lab to compare his results with other family DNA surname groups. This presented us with a very interesting development. Roger's profile matched perfectly, 12 of 12 markers, with two people outside of our group with the surname Ivey. Family Tree DNA provided their email addresses in the report to Roger and the administrators, in case we wanted to get in touch with them. Wayne immediately emailed Jerry Lee Ivey and he, Jerry, is very interested in further research to clarify this relatedness. For background, Roger and Wayne are first cousins once removed and Roger is an historian, and is very excited about conducting further study to verify this connection. Gaila, Roger and Wayne will pursue this latest finding and keep the group informed. With some cursory research online, Pat and Wayne found many Iveys living in the same southern states as the Busby family, and with similar migration patterns. This is the procedure for participants to follow to allow matches of their results with other surname group participants: go to the website for Family Tree DNA. Enter your Kit Number and Privacy Code. On your personal data page, click on Update Contact Information. Under your name and address, uncheck Private: Restrict Match Notification to Your Surname Project. Click Update at the bottom of the page. To further explore the family origins, Roger has ordered additional tests including the Y-DNARefine test (25 markers). When you compare the 12 marker result to someone else who does not have the same surname, but the scores match, you are most likely not recently related - meaning, within the last 1000 years, or 40 generations. Exceptions to this are if an unannounced adoption or false paternity has occurred. The range of generations for the common ancestor extends to 76.9 generations, or about 2000 years for those cases where there is not a surname in common. The surname project is ongoing, and the administrators are hoping for more participants to come forward. Contact Wayne Busbice at "[email protected]"for information. __________________________________________ Late bulletin just received from Michael Lee Busby: Family Tree DNA just announced that Michael has an exact match in his 12 marker Y-DNA test outside the Busby surname group by the name of Benjamin Miller. Michael is following up on this development to see who the common ancestor might be and he will keep us posted. WEB