The one lady still didn't understand about the negative strand; and she decided it didn't matter. It pretty much doesn't; but I think Ann T said 23 reports the positive strand; and they don't always report it that way so I'll take a shot at it. We hear that there are 2 strands of DNA at a given chromosome and that's true in 1 sense. But there are actually 4 strands. 2 of those strands are never transcribed because they're sort of mirror images. In principle they could have been transcribed; but God and evolution never created the appropriate ribosome for reading and transcribing them (or if It did create 1, it didn't survive). re the other point. 23 tells us whether they're reporting a + or - .